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Updated: Jun 13, 2026
Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum
Published on: December 13, 2017
Analytical performance of a sensitive assay for cardiac troponin I with loci technology
M M Arrebola1, J A Lillo, M J Diez De Los Ríos
1Department of Biochemistry and Core Laboratory, Carlos Haya Regional University Hospital, Avda Carlos Haya s/n, 29010 Málaga, Spain. arrebola99@yahoo.es
Objectives:
To confirm the analytical performance of the Dimension Vista LOCI troponin I assay (cTnI).
Design And Method:
Limit of blank (LoB), limit of detection (LoD), limit of quantitation (LoQ) with a 10% coefficient of variation (CV), linearity, precision, method comparison, and 99th percentile upper reference limits (URL) were analyzed. Endogenous analytes and rheumatoid factor (RF) were tested for assay interference.
Results:
The 99th percentile was 0.022 microg/L (CV=14%) and the LoQ was 0.036 microg/L. The ratio of 10% CV concentration to 99th percentile was 1.63. Linearity extended from 0 to 44.36 microg/L. The method comparison equation was Dimension(R) Vista=0.94 (Dimension RxL)+0.00 microg/L with bias at low levels. No interference was detected.
Conclusions:
This study shows acceptable performance characteristics of the LOCI cTnI assay on Dimension Vista to diagnosis and risk stratification of patients with acute coronary syndrome symptoms.
