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Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns)
Published on: April 6, 2019
Serum proteinogram of cats experimentally infected by Trypanosoma evansi
Márcio Machado Costa1, Aleksandro Schafer da Silva, Patrícia Wolkmer
1Small Animals Department, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. marmcvet@yahoo.com.br
Abstract:
This study was aimed at evaluating the electrophoretic profile of serum proteins in Trypanosoma evansi-infected cats during different periods of infection. Thirteen adult females non-breeding Felis catus were separated into two groups. Animals from the infected group (n=7) were inoculated intraperitoneally with a strain of T. evansi; whereas, animals from the control group (n=6) received a physiological solution. Blood samples were collected at days 0, 7, 21, and 35 for total protein evaluation and protein fractionation by electrophoresis. Albumin (P<0.01), alpha-2 globulin and gamma globulin (P<0.05) concentrations were statistically different from the seventh day post-inoculation onwards. Beta-globulin levels were increased from day 21 onwards (P<0.05). Alpha-1 globulin fraction did not differ statistically. These results indicate that the infection by T. evansi in cats alters the serum protein electrophoretic profile.
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