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Updated: Jun 13, 2026
Microcrystallography of Protein Crystals and In Cellulo Diffraction
Published on: July 21, 2017
Crystallization and preliminary X-ray crystallographic analysis of MinE, the cell-division topological specificity
Gil Bu Kang1, Hye Eun Song, Mun Kyoung Kim
1Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea.
Abstract:
Cell division in Gram-negative bacteria is driven by the formation of an FtsZ ring at the division site. MinE regulates the proper placement of the FtsZ ring at mid-cell by blocking the inhibitory action of the MinCD complex. Diffraction data were collected at 2.8 A resolution from a native crystal of full-length Helicobacter pylori MinE. The crystal belonged to space group P6(4). Assuming the presence of two molecules in the asymmetric unit, the calculated Matthews coefficient was 2.58 A(3) Da(-1), which corresponds to a solvent content of 52.3%. For MAD phasing, a four-wavelength data set was collected at 3.0 A resolution.
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