Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Chromatin Structure and RNA Splicing

Chromatin Structure and RNA Splicing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Cosine Similarity Distillation Vision Mixture-of-Experts for Intelligent Housing-Dimensional Urban Physical Examinations.

Sensors (Basel, Switzerland)·2026

Same author

Comparative assessing prefabricated and conventional 3D morphology carbon emission intensity of economy chain hotel buildings in Hangzhou.

Scientific reports·2026

Same author

lncRNA-operated stem cell fate control: RPS3 mediates the in situ Gm16751-Zhx3 interactome to program spermatogonial stem cell differentiation.

Cell & bioscience·2026

Same author

An in vivo parallelized reporter assay to uncover tissue-specific splicing regulatory sequences in a multicellular animal.

Nucleic acids research·2026

Same author

Carbon reduction multidimensional ternary model for robotic 3D printing prefabricated construction across hotels star levels.

Carbon balance and management·2026

Same author

An X-linked long non-coding RNA, PTCHD1-AS, and the core features of autism.

Nature·2026

Same journal

Daily briefing: 'Cyborg' cockroaches breathe underwater with printed suit.

Nature·2026

Same journal

China boosts prestigious grants for young scientists - will it ease competition?

Nature·2026

Same journal

Incoming US science academy chief vows to 'double down' on research.

Nature·2026

Same journal

Author Correction: Synthesis of enantioenriched atropisomers by biocatalytic deracemization.

Nature·2026

Same journal

Electrodeposited self-assembled molecules for perovskite photovoltaics.

Nature·2026

Same journal

Neutrino's nursery found: the 'Shadow Blaster'.

Nature·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 13, 2026

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Deciphering the splicing code.

Yoseph Barash¹, John A Calarco, Weijun Gao

¹Biomedical Engineering, Department of Electrical and Computer Engineering, University of Toronto, 10 King's College Road, Toronto M5S 3G4, Canada.

|May 7, 2010

Summary

This summary is machine-generated.

Scientists developed a splicing code using RNA features to predict tissue-specific alternative splicing. This code reveals novel splicing patterns and regulatory mechanisms, aiding disease research.

More Related Videos

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Related Experiment Videos

Last Updated: Jun 13, 2026

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Area of Science:

Molecular Biology
Genomics
Bioinformatics

Background:

Alternative splicing is vital for biological complexity.
Misregulation of alternative splicing is linked to human diseases.

Purpose of the Study:

To assemble a predictive 'splicing code' for tissue-dependent alternative splicing.
To identify novel splicing patterns and regulatory programs.
To discover mutation-verified regulatory sequences.

Main Methods:

Development of a computational model ('splicing code').
Analysis of hundreds of RNA features to predict exon splicing.
Genome-wide analysis of alternative splicing events.

Main Results:

The code predicts tissue-specific alternative splicing for thousands of exons.
Identified new classes of splicing patterns and distinct tissue-specific regulatory programs.
Revealed widespread regulatory strategies, including intronic features and transcript structure modulation.
Discovered exons that silence expression in adults via nonsense-mediated decay but promote expression during embryogenesis.

Conclusions:

The 'splicing code' provides a powerful tool for understanding alternative splicing regulation.
Facilitates genome-wide discovery and characterization of regulated alternative splicing events.
Offers insights into the role of alternative splicing in development and disease.