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Published on: November 29, 2011
Interaction of Estrogen Receptor Associated Protein (ERAP) 140 with ER beta decreases but its expression increases in
1Biochemistry and Molecular Biology Laboratory, Centre of Advanced study, Department of Zoology, Banaras Hindu University, Varanasi, 221 005, India.
Abstract:
Following binding to cognate ligand, estrogen receptor (ER) beta interacts with specific responsive elements of the target genes and recruits a host of nuclear proteins for hormone dependent gene regulation. However, it is poorly known which proteins interact with ER beta in mouse brain and whether their interaction and expression change with age. In this report, we have used his-tag mouse ER beta for interaction with nuclear proteins of cerebral cortex of young (6 +/- 1 weeks), adult (25 +/- 2 weeks), and old (70 +/- 5 weeks) female mice. We have identified estrogen receptor-associated protein (ERAP) 140 as one of the interacting proteins and studied its interaction by pull down immunoblotting, far-Western blotting and immunoprecipitation, and expression by western blotting. The data show that ERAP 140 interacts with ER beta and its interaction decreases but its expression increases with age in mouse cerebral cortex, suggesting its role in estrogen-mediated brain functions during aging.
Insights
Estrogen receptor-associated protein 140 (ERAP 140) interacts with estrogen receptor beta (ER beta) in the aging mouse brain. ERAP 140 expression increases while its interaction with ER beta decreases with age.
Area of Science:
- Neuroscience
- Endocrinology
- Molecular Biology
Background:
- Estrogen receptor beta (ER beta) regulates gene expression in the brain through protein interactions.
- Little is known about ER beta interacting proteins in the mouse brain and their age-related changes.
Purpose of the Study:
- To identify proteins interacting with ER beta in the aging mouse cerebral cortex.
- To investigate age-dependent changes in the interaction and expression of identified proteins.
Main Methods:
- Used his-tag mouse ER beta to pull down interacting nuclear proteins from young, adult, and old female mice.
- Identified estrogen receptor-associated protein 140 (ERAP 140) as an interacting protein.
- Analyzed ERAP 140-ER beta interaction using pull-down immunoblotting, far-Western blotting, and immunoprecipitation.
- Assessed ERAP 140 expression levels via Western blotting.
Main Results:
- Estrogen receptor-associated protein 140 (ERAP 140) was identified as a protein interacting with ER beta.
- ERAP 140 interaction with ER beta decreased with increasing age in the mouse cerebral cortex.
- ERAP 140 expression levels increased with age in the mouse cerebral cortex.
Conclusions:
- ERAP 140 is an age-dependent interacting protein of ER beta in the mouse brain.
- The inverse relationship between ERAP 140 expression and its interaction with ER beta suggests a role in estrogen-mediated brain functions during aging.
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