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Related Concept Videos

Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
Membrane Domains01:18

Membrane Domains

The membrane domains concentrate specific lipids and proteins at one place within the membrane, which helps in cell signaling, adhesion, and other critical cellular processes. These domains can differ in size, composition, function, and lifespan.
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the anterior...

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Related Experiment Video

Updated: Jun 13, 2026

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method
07:56

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method

Published on: May 8, 2014

Electrically addressable, biologically relevant surface-supported bilayers.

Janice Lin1, John Szymanski, Peter C Searson

  • 1Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA.

Langmuir : the ACS Journal of Surfaces and Colloids
|May 8, 2010
PubMed
Summary
This summary is machine-generated.

This study demonstrates a reproducible method for creating robust, biologically relevant lipid bilayers on silicon substrates. These planar supported bilayers are suitable for membrane research and can incorporate charged lipids for studying cationic peptides.

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Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

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Related Experiment Videos

Last Updated: Jun 13, 2026

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method
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Published on: May 8, 2014

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Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

Area of Science:

  • Biophysics
  • Materials Science
  • Membrane Biology

Background:

  • Vesicular lipid systems are crucial in biological research but lack experimental versatility.
  • Electrically addressable planar supported bilayers offer enhanced capabilities for membrane and membrane protein research.
  • Developing reproducible methods for constructing these bilayers with physiologically relevant lipids is essential.

Purpose of the Study:

  • To assess the electrical properties of planar supported lipid bilayers composed of various physiologically relevant lipids.
  • To demonstrate the reproducible construction of robust, high-resistance bilayers using 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and cholesterol.
  • To enable studies of pore-forming peptides by incorporating charged lipids into the bilayer's outer leaflet.

Main Methods:

  • Fabrication of planar supported lipid bilayers on atomically smooth, single-crystal silicon substrates.
  • Utilizing a polymer cushion for natural incorporation of membrane proteins.
  • Characterization of electrical properties and reproducibility of bilayers with varying lipid compositions, including POPC and cholesterol, and charged lipids.

Main Results:

  • Demonstrated high reproducibility in constructing robust, biologically relevant planar supported bilayers with high electrical resistance.
  • Successfully formed bilayers using 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 25 mol % cholesterol.
  • Incorporated up to 10 mol % negatively charged lipids into outer leaflets for studying cationic pore-forming peptides.

Conclusions:

  • The developed platform enables the reproducible assembly of electrically addressable, planar supported lipid bilayers with high resistance.
  • The unique features, including a commercially available silicon substrate, polymer cushion for protein incorporation, and high reproducibility, advance membrane research.
  • This system is well-suited for investigating membrane properties and the behavior of membrane proteins, including pore-forming peptides.