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Updated: Jul 10, 2026

Preparation, Imaging, and Quantification of Bacterial Surface Motility Assays
Published on: April 7, 2015
Form and functions of the regular surface array (S-layer) of Aeromonas salmonicida
1Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada.
Abstract:
The principal virulence factor of Aeromonas salmonicida is its S-layer (A-layer) which is comprised of tetragonally arrayed approximately 50,000 Mr protein subunits tethered to the cell surface via LPS. The detailed composition of its LPS is known, as is the primary sequence, and three-dimensional disposition of the A protein subunits. The A-layer physically protects the cell against bacteriophage, proteases, as well as immune and non-immune complement. The A-layer appears to be uniquely adapted towards binding biologically important molecules such as heme, and to various basement membrane proteins. In addition, the A-layer is required for macrophage infiltration and resistance. Specific mutants containing a disorganized A-layer are avirulent and provide significant protection to salmonids when applied by immersion.
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