Related Experiment Videos
rig encodes ribosomal protein S15. The primary structure of mammalian ribosomal protein S15
M Kitagawa1, S Takasawa, N Kikuchi
1Department of Biochemistry, Tohoku University School of Medicine, Miyagi, Japan.
Abstract:
rig, a gene originally isolated from a rat insulinoma cDNA library, codes for a basic 145 amino acid protein [( 1986) Diabetes 35, 1178-1180]. Here we show that the immunoreactivity to a monoclonal antibody against the deduced rig protein and the translation product of rig mRNA comigrated with ribosomal protein S15. The amino acid sequence of ribosomal protein S15 purified from rat liver coincided with that deduced from the nucleotide sequence of rig mRNA, but there were indications that the initiator methionine was removed and the succeeding alanyl residue was monoacetylated. From these results, we conclude that the product of rig is ribosomal protein S15.
Insights
The rig gene product was identified as ribosomal protein S15. This finding clarifies the function of the rig gene, originally isolated from rat insulinoma cells.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- The rig gene was initially isolated from a rat insulinoma cDNA library.
- It was known to encode a basic 145 amino acid protein.
Purpose of the Study:
- To identify the protein product of the rig gene.
- To determine the precise function and identity of the rig gene product.
Main Methods:
- Immunoreactivity analysis using a monoclonal antibody against the deduced rig protein.
- Comparison of the translation product of rig mRNA with known ribosomal proteins.
- Amino acid sequencing of purified rat liver ribosomal protein S15.
- Comparison of the amino acid sequence of ribosomal protein S15 with the sequence deduced from rig mRNA.
Main Results:
- The immunoreactivity of the rig protein and its mRNA translation product comigrated with ribosomal protein S15.
- The amino acid sequence of purified rat liver ribosomal protein S15 matched the sequence deduced from rig mRNA.
- Evidence suggested post-translational modification, including removal of the initiator methionine and monoacetylation of the subsequent alanyl residue.
Conclusions:
- The product of the rig gene is ribosomal protein S15.
- The rig gene encodes a key component of the ribosome, essential for cellular function.