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Related Concept Videos

Disorders of Leukocytes01:27

Disorders of Leukocytes

Leukocyte disorders can lead to either leukopenia, characterized by an abnormally low leukocyte count, or leukocytosis, marked by a very high leukocyte number.
Leukopenia may result from bone marrow disorders, autoimmune diseases, and infectious diseases. For example, conditions such as multiple myeloma and aplastic anemia can impair the bone marrow's ability to produce adequate leukocytes. Similarly, autoimmune diseases like lupus and viral infections such as HIV can prompt the immune system...

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Related Experiment Video

Updated: Jun 13, 2026

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
09:12

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Published on: October 17, 2018

Human monocyte heterogeneity--a nephrological perspective.

Kyrill S Rogacev1, Gunnar H Heine

  • 1Saarland University Hospital, Department of Internal Medicine IV, Nephrology and Hypertension, 66421 Homburg, Germany.

Nephrologie & Therapeutique
|May 8, 2010
PubMed
Summary
This summary is machine-generated.

CD16+ monocytes, often called proinflammatory monocytes, are linked to poor cardiovascular outcomes in end-stage renal disease patients. Dialysis temporarily reduces these monocytes, but their levels and changes predict patient health.

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Related Experiment Videos

Last Updated: Jun 13, 2026

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
09:12

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Published on: October 17, 2018

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions
09:41

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Published on: October 17, 2017

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
09:16

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Published on: November 26, 2018

Area of Science:

  • Immunology
  • Hematology

Background:

  • Monocytes are crucial innate immune cells and precursors to tissue macrophages.
  • Human monocytes are heterogeneous, classified into three subsets based on CD14 and CD16 expression: CD14++CD16-, CD14++CD16+, and CD14(+)CD16+.
  • CD16+ monocytes (the latter two subsets) are elevated in inflammatory states like end-stage renal disease (ESRD).

Purpose of the Study:

  • To investigate the functional differences and pathophysiological roles of distinct human monocyte subsets.
  • To explore the significance of CD16+ monocyte kinetics in ESRD patients undergoing dialysis.

Main Methods:

  • Flow cytometry was used to distinguish and quantify monocyte subsets based on CD14 and CD16 expression.
  • Analysis of monocyte counts before and after dialysis sessions in ESRD patients.
  • Correlation analysis between monocyte subset levels/kinetics and cardiovascular outcomes.

Main Results:

  • Each dialysis session causes a transient decrease in CD16+ monocytes (monocytopenia).
  • Elevated predialysis counts of CD16+ monocytes are associated with adverse cardiovascular outcomes in ESRD.
  • Dialysis-induced changes in CD16+ monocyte counts also predict cardiovascular events.

Conclusions:

  • CD16+ monocyte counts and their dynamic changes during dialysis are significant predictors of cardiovascular risk in ESRD.
  • Further research is needed to fully elucidate the functional distinctions and precise roles of monocyte subsets in disease pathogenesis.