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Related Experiment Video

Updated: Jun 13, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)

Published on: March 14, 2013

Global metabolic profiling procedures for urine using UPLC-MS.

Elizabeth J Want1, Ian D Wilson, Helen Gika

  • 1Biomolecular Medicine, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, UK. e.want@imperial.ac.uk

Nature Protocols
|May 8, 2010
PubMed
Summary
This summary is machine-generated.

Ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) enhances urinary metabolite profiling by improving coverage. This method offers superior metabolome insights for studying diseases and toxic insults.

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A Strategy for Sensitive, Large Scale Quantitative Metabolomics
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A Strategy for Sensitive, Large Scale Quantitative Metabolomics

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Related Experiment Videos

Last Updated: Jun 13, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)

Published on: March 14, 2013

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

Published on: May 20, 2013

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

Area of Science:

  • Metabolomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Global metabolite profiling analyzes low molecular-weight metabolites (<1 kDa) in biofluids to understand physiological responses.
  • Mass spectrometry (MS) is crucial for generating informative metabolite profiles.
  • Studying metabolic perturbations aids in disease diagnosis and toxicology.

Purpose of the Study:

  • To detail the application of ultra-performance liquid chromatography-MS (UPLC-MS) for comprehensive urinary metabolite profiling.
  • To optimize sample preparation, stability, storage, and chromatographic conditions for UPLC-MS analysis.
  • To provide a framework for quality control, metabolite identification, and data analysis in UPLC-MS studies.

Main Methods:

  • Development of a UPLC-MS protocol for urinary metabolite profiling.
  • Optimization of sample preparation, chromatographic separation, and data processing.
  • Implementation of quality control measures and multivariate data analysis.

Main Results:

  • The UPLC-MS protocol provides superior metabolome coverage compared to conventional HPLC methods.
  • Analysis of a 96-well plate sample set takes approximately 30 hours, including setup, preparation, analysis, and processing.
  • The method balances metabolome coverage, chromatographic resolution, and analytical throughput.

Conclusions:

  • UPLC-MS is a powerful technique for detailed urinary metabolite profiling.
  • This protocol enables enhanced understanding of metabolic changes in health and disease.
  • The improved chromatographic performance leads to more comprehensive metabolic data acquisition.