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Related Experiment Video

Updated: Jun 13, 2026

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors

Published on: September 10, 2013

Ratiometric pH-dot ANSors.

Xiaojuan Wang¹, Chiara Boschetti, Maria Jose Ruedas-Rama

¹Institute of Biotechnology, Department of Chemical Engineering and Biotechnology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QT, UK.

|May 8, 2010

Summary

This summary is machine-generated.

A novel silica nanosphere sensor (ANSor) with quantum dots (QDs) offers ratiometric pH measurement. This robust sensor accurately detects local pH in physiologically relevant ranges.

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Area of Science:

Nanotechnology
Materials Science
Analytical Chemistry

Background:

Accurate local pH measurement is crucial in biological and chemical systems.
Existing pH sensors may have limitations in stability, robustness, or sensitivity.

Purpose of the Study:

To develop a silica-based analytical nanosphere sensor (ANSor) for ratiometric pH measurement.
To create a robust and stable sensor capable of operating in physiologically relevant pH ranges.

Main Methods:

Quantum dots (QDs) were embedded within a silica matrix using the Stöber method.
A pH-responsive QD and a reference QD were incorporated into the silica nanospheres.
Ratiometric pH estimation was achieved by exciting both QD populations simultaneously and analyzing their emission spectra.

Main Results:

Highly fluorescent QD-embedded silica particles were synthesized with high yield.
The developed ANSor demonstrated stable and robust performance.
The sensor accurately measured pH in the physiologically relevant range.

Conclusions:

The silica-based analytical nanosphere sensor (ANSor) provides a reliable method for ratiometric pH sensing.
This QD-based sensor offers a promising tool for local pH monitoring in various applications.
The sensor's stability and robustness make it suitable for complex environments.