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Published on: November 10, 2023
[Establishment of one-step reverse transcription-polymerase chain reaction method for mumps virus hydrophobin gene
Xin Chang1, Jian-Hui Zhou, Shuang Wang
1Jilin Provincial Center for Disease Control and Prevention, Key Laboratory of Health Bureau of Jilin Province, Changchun 130062, Jilin, China.
Objective:
To establish a kind of simple method for identifying mumps virus gene: one-step reverse transcription-polymerase chain reaction, RT-PCR).
Methods:
To design primer in both sides of SH gene, and then to do the sensitivity and specificity test for the RT-PCR method.
Results:
At least 10(0.7) TCID50 mumps virus can be detected by this method. 5 strains of mumps virus can be detected and 4 strains of non-mumps virus including measles and rubella virus can't be detected by this method.
Conclusions:
The one-step RT-PCR method is a rapid and simple method for identifying mumps virus gene.
