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Updated: Jun 13, 2026

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
Published on: October 23, 2011
[Detection of bacterial meningitis by multiplex polymerase chain reaction]
Hong-Yu Ren1, Shu-Yi An, Ma-Chao Li
1State Key Laboratory for Infectious Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Objective:
To develop a rapid method for detecting 8 pathogens which were highly related to bacterial meningitis by multiplex polymerase chain reaction.
Method:
By optimizing the reaction condition and amplification program of single pair polymerase chain reaction, the multiplex pairs polymerase chain reactions (M-PCR) was developed to identify eight pathogens simultaneously including Neisseria Meningitis, Haemophilus Influenzae, Streptococcus Pneumoniae, Cryptococcus Neoformans, Staphylococcus Aureus, Listerisa Monocytogene, Streptococcus Suis and Mycobacterium Tuberculosis. Meanwhile, The sensitivity of M-PCR assay was also studied.
Results:
M-PCR methods for detecting 8 pathogens which could cause bacterial meningitis have been established. M-PCR showed specific, sensitive and more rapid than conventional culturing method.
Conclusion:
This multiplex polymerase chain reaction method can be used for diagnosis and scanning of suspicious bacterial meningitis cases in order to improve the diagnostic positive rate of bacterial meningitis cases.
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Bacterial Meningitis I: Introduction
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