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Assignment of the human pancreatic colipase gene to chromosome 6p21.1 to pter
R C Davis1, Y R Xia, T Mohandas
1Lipid Research, VA Wadsworth Medical Center, Los Angeles, California 90073.
Insights
Researchers identified the human colipase gene (CLPS) location using molecular techniques. This gene is crucial for pancreatic lipase function in dietary fat absorption.
Area of Science:
- Biochemistry
- Molecular Biology
- Genetics
Background:
- Pancreatic colipase is a vital cofactor for pancreatic lipase, facilitating dietary long-chain fatty acid absorption.
- Colipase stabilizes lipase on lipid micelles, opposing bile salt interference during digestion.
Purpose of the Study:
- To clone the complete coding region of human procolipase mRNA.
- To determine the chromosomal location of the human colipase gene (CLPS).
Main Methods:
- Polymerase chain reaction (PCR) was used to generate a cDNA clone of human procolipase mRNA.
- Southern blot analysis of mouse-human somatic cell hybrids identified the gene's chromosome.
- Analysis of translocation hybrids refined the gene's regional localization on chromosome 6.
Main Results:
- A cDNA clone representing the full coding sequence of human procolipase mRNA was successfully produced.
- The colipase gene (CLPS) was mapped to human chromosome 6.
- CLPS was regionally localized to the 6p21.1-pter region of chromosome 6.
Conclusions:
- The study successfully cloned human procolipase cDNA and localized the CLPS gene to chromosome 6p21.1-pter.
- This provides a genetic basis for understanding colipase function and potential related disorders.
Abstract:
Pancreatic colipase is a 12-kDa polypeptide cofactor for pancreatic lipase (EC 3.1.1.3), an enzyme essential for the absorption of dietary long-chain triglyceride fatty acids. Colipase is thought to anchor lipase noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. Using primers derived from the known amino acid sequence, we have used the polymerase chain reaction to produce a cDNA clone corresponding to the complete coding region of the human procolipase mRNA. Southern blot analysis of genomic DNA from a panel of mouse-human somatic cell hybrids indicated that the colipase gene (CLPS) resides on human chromosome 6. Further analysis of somatic cell hybrids carrying chromosome 6 translocations permitted regional localization of CLPS to the 6p21.1-pter region.
