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Updated: Jun 13, 2026

Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
Packing behaviour of two predominant anionic phospholipids of bacterial cytoplasmic membranes
Florian Prossnigg1, Andrea Hickel, Georg Pabst
1Institute of Biophysics and Nanosystems Research, Austrian Academy of Sciences, Schmiedlstrasse 6, A-8042 Graz, Austria.
Abstract:
Phosphatidylglycerol and cardiolipin represent the most abundant anionic phospholipid components of cytoplasmic bacterial membranes and thus are used as constituents for membrane mimetic systems. In this study, we have characterized the temperature dependent phase behaviour of the binary system dipalmitoyl-phosphatidylglycerol (DPPG) and tetramyristoyl-cardiolipin (TMCL) using microcalorimetry and X-ray scattering techniques. Both lipids exhibited a very similar main transition temperature ( approximately 41 degrees C), showing a minimum (39.4 degrees C) for the binary mixtures at X(DPPG)=0.8, and exhibited low-temperature phase transitions, which were abolished by incorporation of small amounts (
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