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Updated: Jun 13, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
Published on: July 8, 2025
Singly and doubly labeled base-discriminating fluorescent oligonucleotide probes containing oxo-pyrene chromophore
Subhendu Sekhar Bag1, Rajen Kundu, Katsuhiko Matsumoto
1Bioorganic Chemistry Laboratory, Department of Chemistry, Indian Institute of Technology, Guwahati 781039, Assam, India. ssbag75@iitg.ernet.in
Abstract:
We have developed new oxo-pyrene labeled fluorescent nucleoside, (Oxo-Py)U which showed a strong fluorescence dependency on solvent polarity at long wavelength. The designed singly and doubly (Oxo-Py)U labeled fluorescent oligonucleotide probes were found highly efficient for the discrimination of A and consecutive AA bases of target DNA opposite to the labeled base via generation of enhanced fluorescence signal.
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