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Marco Agostini1, Salvatore Pucciarelli, Federica Calore

  • 1Clinica Chirurgica II, Dipartimento Scienze Oncologiche e Chirurgiche, Università di Padova, Italy. m.agostini@unipd.it

Clinica Chimica Acta; International Journal of Clinical Chemistry
|May 11, 2010
PubMed

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MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...