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Updated: Jun 13, 2026

Reconstitution of Membrane-Tethered Minimal Actin Cortices on Supported Lipid Bilayers
Published on: July 12, 2022
Atomic force microscopy imaging of the structure of the motor protein prestin reconstituted into an artificial lipid
Shun Kumano1, Michio Murakoshi, Koji Iida
1Department of Bioengineering and Robotics, Tohoku University, Sendai, Japan.
Abstract:
Prestin is the motor protein of cochlear outer hair cells and is essential for mammalian hearing. The present study aimed to clarify the structure of prestin by atomic force microscopy (AFM). Prestin was purified from Chinese hamster ovary cells which had been modified to stably express prestin, and then reconstituted into an artificial lipid bilayer. Immunofluorescence staining with anti-prestin antibody showed that the cytoplasmic side of prestin was possibly face up in the reconstituted lipid bilayer. AFM observation indicated that the cytoplasmic surface of prestin was ring-like with a diameter of about 11 nm.

