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Updated: Jun 13, 2026

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues
Published on: November 22, 2014
Oligonucleotide-selenide conjugate: synthesis and its inducible sequence-specific alkylation of DNA
Yuhao Du1, Xiaocheng Weng, Jing Huang
1Wuhan University, Hubei, Wuhan 430072, People's Republic of China.
Abstract:
Oligonucleotide-selenium conjugate was designed and synthesized and its sequence-specific cross-linking ability was investigated. The selenide derivatives can generate covalent interstrand cross-linking with its complementary strand through the formation of o-QM intermediate induced by periodate oxidation. A cross-linking reaction yield of up to 50% was obtained. Hydroxyl radical footprinting experiment revealed that the quinone appendage specifically alkylated the cytosine base extending the duplex formed between the conjugate and the target strand.
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