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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Riboswitches01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

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Related Experiment Video

Updated: Jun 13, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Basic and clinical studies on functional RNA molecules for advanced medical technologies.

Toshihiro Takizawa1, Akihiko Gemma, Kumiko Ui-Tei

  • 1Division of Molecular Medicine and Anatomy, Graduate School of Medicine, Nippon Medical School, Tokyo, Japan. t-takizawa@nms.ac.jp

Journal of Nippon Medical School = Nippon Ika Daigaku Zasshi
|May 11, 2010
PubMed
Summary

Small interfering RNAs (siRNAs) and microRNAs (miRNAs) regulate gene expression. This review covers siRNA functions, miRNA research in the human placenta, and miRNA applications in lung cancer, exploring functional RNA molecules.

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Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions
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Last Updated: Jun 13, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
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Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions
11:57

Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions

Published on: April 21, 2016

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Small interfering RNAs (siRNAs) and microRNAs (miRNAs) are key regulators of gene expression.
  • RNA interference (RNAi) mediated by siRNAs is a powerful tool for gene knockdown in research.
  • Endogenous miRNAs play critical roles in human physiological and pathological processes.

Purpose of the Study:

  • To review the synthesis, mechanisms, and functions of siRNAs.
  • To highlight recent advances in miRNA and protein research of the human placenta.
  • To discuss clinical applications of miRNAs in lung cancer and touch upon long noncoding RNAs.

Main Methods:

  • Literature review and synthesis of existing research.
  • Focus on functional RNA molecules and their regulatory roles.
  • Exploration of technological advancements in miRNA and protein research.

Main Results:

  • siRNAs are effective tools for elucidating gene function and disease mechanisms.
  • miRNAs are implicated in diverse biological processes and diseases.
  • Emerging research explores placental miRNA/protein interactions and therapeutic miRNA strategies for lung cancer.

Conclusions:

  • Functional RNA molecules like siRNAs and miRNAs offer significant potential in basic research and clinical applications.
  • Further research into miRNAs is crucial for understanding human diseases and developing novel therapies.
  • Long noncoding RNAs represent another area of growing interest in genomic research.