Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

12.1K
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
12.1K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

8.5K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
8.5K
Homologous Recombination02:31

Homologous Recombination

58.8K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
58.8K
Gene Conversion02:08

Gene Conversion

9.2K
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
9.2K
DNA Isolation01:24

DNA Isolation

35.3K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
35.3K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

2.4K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
2.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

NELF prevents transcriptional readthrough into DNA replication zones in cancer cells.

EMBO reports·2026
Same author

Regulated TRESLIN-MTBP loading governs initiation zones and replication timing in human DNA replication.

Nature communications·2025
Same author

Rad53 regulates RNase H1, which promotes DNA replication through sites of transcription-replication conflict.

Cell reports·2025
Same author

The INO80 chromatin remodeller facilitates DNA damage bypass via postreplicative gap repair.

The EMBO journal·2025
Same author

Allosteric activation of the SPRTN protease by ubiquitin maintains genome stability.

Nature communications·2025
Same author

Reversible association of ubiquitin with PCNA is important for template switching in S. cerevisiae.

DNA repair·2025

Related Experiment Video

Updated: May 2, 2026

Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks
12:19

Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks

Published on: November 10, 2016

7.7K

Ubiquitin-dependent DNA damage bypass is separable from genome replication.

Yasukazu Daigaku1, Adelina A Davies, Helle D Ulrich

  • 1Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms EN6 3LD, UK.

Nature
|May 11, 2010
PubMed
Summary

Post-replication repair (PRR) uses ubiquitylation of PCNA to bypass DNA damage. Translesion synthesis is the primary mutagenic pathway, with error-free repair acting as a backup system.

More Related Videos

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

2.8K
Capturing Common Fragile Site Breaks by Native γH2A.X ChIP
09:46

Capturing Common Fragile Site Breaks by Native γH2A.X ChIP

Published on: January 24, 2025

707

Related Experiment Videos

Last Updated: May 2, 2026

Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks
12:19

Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks

Published on: November 10, 2016

7.7K
Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

2.8K
Capturing Common Fragile Site Breaks by Native γH2A.X ChIP
09:46

Capturing Common Fragile Site Breaks by Native γH2A.X ChIP

Published on: January 24, 2025

707

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Post-replication repair (PRR) is crucial for cells to overcome DNA lesions during replication.
  • Ubiquitylation of the PCNA protein by the RAD6 pathway activates damage bypass in eukaryotes.
  • Monoubiquitylation enables mutagenic translesion synthesis, while polyubiquitylation supports error-free repair.

Purpose of the Study:

  • To investigate the timing and spatial relationship of PRR events relative to DNA replication forks.
  • To characterize the downstream factors required for PCNA ubiquitylation.
  • To visualize and quantify productive PRR tracts during DNA damage bypass.

Main Methods:

  • Utilized an inducible DNA damage bypass system in Saccharomyces cerevisiae.
  • Synchronized cell populations for precise timing and visualization.
  • Quantified PRR tracts in relation to genome replication.

Main Results:

  • Demonstrated that the RAD6 pathway operates during S phase but can be separated from replication.
  • Showed that ultraviolet-radiation-induced lesions are primarily bypassed via translesion synthesis.
  • Identified the error-free pathway as a backup system for DNA lesion processing.

Conclusions:

  • Developed a novel method for visualizing and quantifying PRR tracts.
  • Revealed the distribution of PRR tracts in synchronized cells.
  • Provides a foundation for mechanistic analysis of lesion processing during and after replication.