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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.

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Updated: Jun 13, 2026

Quantitative Locomotion Study of Freely Swimming Micro-organisms Using Laser Diffraction
10:03

Quantitative Locomotion Study of Freely Swimming Micro-organisms Using Laser Diffraction

Published on: October 25, 2012

Laser fluorescence velocimeter.

D Keefer

    Applied Optics
    |May 11, 2010
    PubMed
    Summary
    This summary is machine-generated.

    A novel laser-induced fluorescence (LIF) technique measures fluid flow velocity using modulated excitation. This method leverages fluorescence signal variations caused by flow, offering a new approach for fluid dynamics research.

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    High-speed Particle Image Velocimetry Near Surfaces
    11:59

    High-speed Particle Image Velocimetry Near Surfaces

    Published on: June 24, 2013

    Area of Science:

    • Fluid dynamics
    • Laser-based measurement techniques
    • Optical diagnostics

    Background:

    • Accurate measurement of fluid flow velocity is crucial in various scientific and engineering fields.
    • Existing techniques like laser Doppler velocimetry (LDV) have limitations.
    • Laser-induced fluorescence (LIF) offers potential for non-intrusive flow measurement.

    Purpose of the Study:

    • To introduce a new technique for determining fluid flow velocity using laser-induced fluorescence (LIF).
    • To describe the principles behind modulated excitation in LIF for flow measurement.
    • To present a model illustrating the technique and its key parameters.

    Main Methods:

    • Utilizing space and time modulated laser excitation to generate LIF signals.
    • Employing a crossed-beam probe volume, similar to LDV systems.
    • Analyzing flow-induced variations in the visibility and phase of the fluorescence signal.

    Main Results:

    • Demonstrated a new method for fluid flow velocity determination via LIF.
    • Identified the dependence of the LIF signal on flow characteristics and fluorescence decay time.
    • Presented a theoretical model to explain the observed phenomena.

    Conclusions:

    • The described LIF technique provides a viable method for non-intrusive fluid flow velocity measurement.
    • The technique's performance is influenced by excitation modulation and the fluorescence lifetime of the species.
    • Further development and validation of the technique are warranted for practical applications.