Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.
Atomic Emission Spectroscopy: Instrumentation01:22

Atomic Emission Spectroscopy: Instrumentation

The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
Atomic Emission Spectroscopy: Lab01:29

Atomic Emission Spectroscopy: Lab

AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
Atomic Absorption Spectroscopy: Radiation and Light Sources01:13

Atomic Absorption Spectroscopy: Radiation and Light Sources

Atomic absorption spectroscopy (AAS) relies on the Beer-Lambert law, which requires that the radiation source emits a narrow range of wavelengths to match the absorption characteristics of the analyte atom. The primary criteria for choosing an appropriate radiation source in AAS is to provide a precise and intense emission at specific wavelengths that will allow accurate detection of the analyte.
Two common narrow-range 'line' sources used in AAS are hollow-cathode lamps (HCLs) and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Progression patterns and clinical outcomes in patients with cutaneous squamous-cell carcinoma following anti-PD-1 therapy failure.

ESMO open·2026
Same author

The invasion pore induced by <i>Toxoplasma gondii</i>.

bioRxiv : the preprint server for biology·2024
Same author

An Automated, Dynamic Radiation Oncology Prescription Checking System.

Practical radiation oncology·2023
Same author

Interferon-induced transmembrane protein 3 (IFITM3) and its antiviral activity.

Current opinion in structural biology·2022
Same author

Weekly paclitaxel, carboplatin, cetuximab, and cetuximab, docetaxel, cisplatin, and fluorouracil, followed by local therapy in previously untreated, locally advanced head and neck squamous cell carcinoma.

Annals of oncology : official journal of the European Society for Medical Oncology·2019
Same author

Resin embedded multicycle imaging (REMI): a tool to evaluate protein domains.

Scientific reports·2016
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jun 13, 2026

High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis
13:31

High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis

Published on: December 22, 2015

Quasielastic light scattering microscope spectrometer.

P S Blank, R B Tishler, F D Carlson

    Applied Optics
    |May 11, 2010
    PubMed
    Summary
    This summary is machine-generated.

    We created a quasielastic light scattering microscope spectrometer. This instrument allows detailed study of microscopic scattering volumes, minimizing spatial averaging for accurate intensity autocorrelation functions.

    More Related Videos

    Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
    15:06

    Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

    Published on: January 3, 2016

    High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis
    07:55

    High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis

    Published on: September 22, 2017

    Related Experiment Videos

    Last Updated: Jun 13, 2026

    High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis
    13:31

    High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis

    Published on: December 22, 2015

    Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
    15:06

    Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

    Published on: January 3, 2016

    High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis
    07:55

    High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis

    Published on: September 22, 2017

    Area of Science:

    • Optics and Photonics
    • Materials Science
    • Biophysics

    Background:

    • Quasielastic light scattering (QLS) is a powerful technique for studying dynamic processes in materials.
    • Microscopic analysis often faces challenges with spatial averaging, affecting signal fidelity.
    • Existing QLS instruments may not meet the coherence requirements for high-resolution microscopic studies.

    Purpose of the Study:

    • To develop a novel quasielastic light scattering microscope spectrometer.
    • To evaluate the coherence performance of the developed system against theoretical predictions.
    • To enable the study of microscopic scattering volumes with reduced spatial averaging.

    Main Methods:

    • Modification of a commercially available instrument to create a QLS microscope spectrometer.
    • Theoretical examination of coherence requirements for microscopic spectrometers.
    • Experimental validation of the system's performance and its agreement with theoretical models.

    Main Results:

    • Successful development of a functional quasielastic light scattering microscope spectrometer.
    • Demonstrated system performance consistent with established theoretical coherence requirements.
    • Capability to analyze microscopic scattering volumes while minimizing spatial averaging effects.

    Conclusions:

    • The developed QLS microscope spectrometer is a viable tool for microscopic dynamic light scattering studies.
    • The system effectively addresses the challenge of spatial averaging in microscopic analyses.
    • This advancement facilitates more precise investigations into the dynamics of small-scale systems.