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Related Concept Videos

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
Total Internal Reflection Fluorescence Microscopy01:05

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...

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Related Experiment Video

Updated: Jun 13, 2026

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
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Absolute reflectometer for the 0.8-2.5-microm region.

D Sheffer, U P Oppenheim, D Clement

    Applied Optics
    |May 11, 2010
    PubMed
    Summary

    A new absolute reflectometer uses an integrating sphere and computer-controlled filters for automatic measurements. It shows unexpected deviations in BaSO(4) reflectivity compared to accepted values.

    Area of Science:

    • Optical physics
    • Spectroscopy
    • Materials science

    Background:

    • Accurate measurement of material reflectance is crucial for various applications.
    • Existing reflectometry methods may require standard surfaces, limiting absolute measurements.
    • The 0.8-2.5 micrometer spectral region is important for understanding material properties.

    Purpose of the Study:

    • To describe a novel absolute reflectometer design.
    • To demonstrate its capability for automatic reflectance measurements.
    • To evaluate the performance of the reflectometer using Barium Sulfate (BaSO4) as a test material.

    Main Methods:

    • Development of an integrating sphere-based reflectometer.
    • Operation in the 0.8-2.5 micrometer spectral range.

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  • Utilizing computer-controlled circular variable filters for automatic wavelength selection.
  • Employing an absolute measurement technique that does not require a reference standard.
  • Main Results:

    • The developed reflectometer provides absolute reflectance values without needing a standard.
    • The system operates automatically, controlled by computer.
    • Measurements of Barium Sulfate (BaSO4) in the near-infrared region revealed significant discrepancies from established reflectivity data.

    Conclusions:

    • The novel absolute reflectometer is a functional and automated system for spectral reflectance measurements.
    • The observed deviations in BaSO4 reflectivity warrant further investigation.
    • This instrument can potentially uncover new insights into material optical properties.