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Fructose-selective calorimetric biosensor in flow injection analysis.

Sunil G Bhand1, Srimathi Soundararajan, Ioana Surugiu-Wärnmark

  • 1Pure and Applied Biochemistry, Centre for Chemistry, Lund University, P.O Box 124, S-221 00 Lund, Sweden. sunil17_bhand@yahoo.com

Analytica Chimica Acta
|May 12, 2010
PubMed
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A novel biosensor accurately measures fructose in syrups without glucose interference. This enzyme thermistor method offers rapid, inexpensive, and reliable fructose analysis for food samples.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Food Science

Background:

  • Accurate fructose quantification is crucial for food analysis.
  • Existing methods may struggle with selectivity and cost-effectiveness.

Purpose of the Study:

  • To develop a highly selective and interference-free biosensor for fructose measurement.
  • To enable rapid and inexpensive fructose analysis in real-world samples like syrups.

Main Methods:

  • Enzyme-based assay utilizing hexokinase and fructose-6-phosphate-kinase.
  • Heat liberation monitored via an enzyme thermistor.
  • Flow Injection Analysis (FIA)-based, mediator-free calorimetric detection.

Main Results:

  • Achieved good linearity (0.5-6.0 mM) and a low detection limit (0.12 mM).

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  • Demonstrated high selectivity, unaffected by glucose.
  • Showcased operational stability over three months and a six-month shelf life.

    • Conclusions:

    • The developed biosensor provides a reliable, rapid, and cost-effective method for routine fructose monitoring.
    • This technique is suitable for analyzing fructose content in commercial food products and syrups.
    • The biosensor's stability and selectivity make it a valuable tool for the food industry.