Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
Autoimmune Disorders01:29

Autoimmune Disorders

Autoimmune diseases are a group of disorders in which the body's immune system mistakenly attacks its own cells, tissues, and organs. This results from an overactive immune response against substances and tissues normally present in the body. Let's delve into the concept and mechanism of autoimmune diseases from an immune system point of view, explore different causes and examples of such diseases, and discuss potential solutions.
Concept and Mechanism of Autoimmune Diseases
The immune system...
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

C5a/C5aR1 mediates AKI-CKD transition by enhancing autophagy.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association·2026
Same author

Beyond B cell helpers: diverse roles of peripheral helper T cells.

International immunology·2026
Same author

Complement 3a Receptor mediates high fat diet induced hypothalamic accumulation of lipid associated microglia to regulate neuroinflammation and obesity.

bioRxiv : the preprint server for biology·2026
Same author

Pathogenic GM-CSF drives functional diversification of inflammatory macrophages in autoimmune arthritis.

Science advances·2026
Same author

Tissue-restricted secondary TCR engagement drives the transition from stem-like to CD200<sup>+</sup> Egr2<sup>hi</sup> arthritogenic Th17 cells.

Nature immunology·2026
Same author

Regnase-1-mediated regulation of neutrophils modulates SARS-CoV-2 pneumonia.

PLoS pathogens·2026

Related Experiment Video

Updated: Jun 13, 2026

Isolation and Th17 Differentiation of Na&iuml;ve CD4 T Lymphocytes
12:59

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Published on: September 26, 2013

Complement drives Th17 cell differentiation and triggers autoimmune arthritis.

Motomu Hashimoto1, Keiji Hirota, Hiroyuki Yoshitomi

  • 1Department of Experimental Pathology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.

The Journal of Experimental Medicine
|May 12, 2010
PubMed
Summary

Complement activation triggers autoimmune disease by promoting Th17 cell expansion. Blocking the C5a receptor (C5aR) suppressed arthritis development in mice, suggesting a therapeutic target for autoimmune conditions.

More Related Videos

In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse
07:46

In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse

Published on: October 25, 2024

Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity
10:10

Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity

Published on: November 8, 2016

Related Experiment Videos

Last Updated: Jun 13, 2026

Isolation and Th17 Differentiation of Na&iuml;ve CD4 T Lymphocytes
12:59

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Published on: September 26, 2013

In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse
07:46

In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse

Published on: October 25, 2024

Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity
10:10

Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity

Published on: November 8, 2016

Area of Science:

  • Immunology
  • Rheumatology
  • Pathology

Background:

  • Serum complement activation is implicated in autoimmune diseases.
  • Th17 cells play a critical role in autoimmune pathogenesis.
  • SKG mice are a genetic model for spontaneous autoimmune arthritis.

Purpose of the Study:

  • To investigate the role of complement activation in Th17 cell-mediated autoimmune disease.
  • To determine the specific contribution of C5a and its receptor (C5aR) in arthritis development.
  • To explore potential therapeutic strategies targeting the complement system.

Main Methods:

  • Administration of complement activators (mannan, beta-glucan) to SKG mice.
  • Assessment of Th17 cell differentiation and expansion.
  • Analysis of cytokine production by macrophages and T cells.
  • Utilizing C5aR-deficient and RAG-deficient mouse models.
  • Macrophage depletion experiments.

Main Results:

  • Complement activation induced Th17 cell-mediated autoimmune arthritis in SKG mice.
  • C5a stimulated macrophages to produce IL-6, enhancing Th17 cell differentiation.
  • C5aR deficiency abrogated Th17 cell expansion and arthritis development.
  • Macrophage depletion also prevented disease onset.

Conclusions:

  • Complement activation, particularly via C5a, is a key initiator of Th17 cell differentiation and expansion in autoimmune settings.
  • Targeting C5a receptor (C5aR) may be a promising therapeutic approach for Th17-mediated inflammatory and autoimmune diseases.