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Parkinson disease (PD) is a progressive neurodegenerative disorder primarily affecting movement, with additional non-motor features. Its pathophysiology involves complex interactions among genetic susceptibility, environmental exposures, and cellular dysfunction, including dopaminergic neuron loss, protein aggregation, and mitochondrial impairment.Selective NeurodegenerationA key feature is the degeneration of dopaminergic neurons in the substantia nigra pars compacta, leading to reduced...

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Related Experiment Video

Updated: Jun 13, 2026

Assaying the Kinase Activity of LRRK2 in vitro

Assaying the Kinase Activity of LRRK2 in vitro

Published on: January 18, 2012

LRRK2 and Parkinson disease.

Justus C Dächsel¹, Matthew J Farrer

¹Laboratories of Neurogenetics, Department of Neuroscience, Morris K. Udall Parkinson's Disease Research Center of Excellence, Mayo Clinic, Jacksonville, Florida 32224, USA.

Archives of Neurology

|May 12, 2010

Summary

Genetic insights into leucine-rich repeat kinase 2 (LRRK2) offer new therapeutic avenues for Parkinson disease (PD). Studying LRRK2 carriers helps define PD

Area of Science:

Neuroscience
Genetics
Molecular Biology

Background:

Leucine-rich repeat kinase 2 (LRRK2) is implicated in parkinsonism.
Genetic mutations in LRRK2 are linked to Parkinson disease (PD).

Purpose of the Study:

To review the molecular genetics and functional biology of LRRK2 in parkinsonism.
To summarize opportunities and challenges for developing PD interventions based on LRRK2 genetics.

Main Methods:

Focused on LRRK2 biology literature from 2004 to March 2009.
Selected original contributions, seminal observations, and thoughtful reviews.
Abstracted data primarily from PubMed.

Main Results:

LRRK2 mutations are diagnostically useful in early or atypical PD presentations.

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Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

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Published on: December 14, 2017

Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson's Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation

Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson's Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation

Published on: March 21, 2020

Related Experiment Videos

Last Updated: Jun 13, 2026

Assaying the Kinase Activity of LRRK2 in vitro

Assaying the Kinase Activity of LRRK2 in vitro

Published on: January 18, 2012

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

Published on: December 14, 2017

Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson's Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation

Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson's Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation

Published on: March 21, 2020

Identified molecular pathways suggest therapeutic interventions for LRRK2 and idiopathic PD.

Opportunities exist to develop relevant biomarkers and experimental models.

Conclusions:

LRRK2 carriers (affected and asymptomatic) enable defining PD's natural history.
This includes frequency, penetrance, and progression of motor and non-motor symptoms.
Associated biomarkers can also be studied in LRRK2 carriers.