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Updated: Jun 13, 2026

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
Published on: February 23, 2016
Properties of dinitroxides for use in dynamic nuclear polarization (DNP)
Cédric Ysacco1, Egon Rizzato, Marie-Alice Virolleaud
1SREP LCP UMR 6264, CNRS et Universités d'Aix Marseille 1, 2 et 3, Faculté de St Jérôme case 521, 13013 Marseille, France.
Abstract:
We have investigated the properties of a series of dinitroxides as polarizing agents for SS NMR/DNP applications. Our results clearly establish that an orthogonal relative orientation of electron g tensors is a crucial requirement to obtain high enhancement DNP factors. Moreover, the ratio of the proton Larmor frequency over the e(-)-e(-) dipolar coupling (omega(H)/omega(D)) influences the efficiency of the cross effect (CE) mechanism, thus the Ree distance between the unpaired electrons must be adapted to omega(H).
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