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Updated: Jun 13, 2026

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells
Published on: June 16, 2019
[Kinetics of growth 2-D and 3-D cell models under influence of microenvironment]
Abstract:
A cocultivation system was developed to investigate the interactions between MCF-7 breast cancer epithelial cells and MT-4 (line human T-cell leukemia) by paracrine mechanism (no contact cocultivation). Viability and proliferation were determined in two fractions of MCF-7 cells from spheroid culture and monolayer. It has been shown that the influence of MT-4 reduced the number of cells MCF-7 in fraction of adhesive cells and did not influence on the suspension fraction. Proliferation was reduced in both fractions without MT-4. Cells viability was different in monolayer and spheroids with MT-4 and without MT-4; in monolayer it was lower, in spheroids it was higher. We think that, MT-4 by paracrine signal way stimulated proliferation MCF 7 cells, reduced adhesive to the bottom and led to MTS formation.
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