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Updated: Jun 13, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
Published on: June 3, 2019
Optical recognition of converted DNA nucleotides for single-molecule DNA sequencing using nanopore arrays
Ben McNally1, Alon Singer, Zhiliang Yu
1Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215, USA.
Abstract:
We demonstrate the feasibility of a nanopore based single-molecule DNA sequencing method, which employs multicolor readout. Target DNA is converted according to a binary code, which is recognized by molecular beacons with two types of fluorophores. Solid-state nanopores are then used to sequentially strip off the beacons, leading to a series of detectable photon bursts, at high speed. We show that signals from multiple nanopores can be detected simultaneously, allowing straightforward parallelization to large nanopore arrays.
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