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Updated: Jun 13, 2026

Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy
Published on: January 7, 2019
Ultrasound-modulated fluorescence from rhodamine B aqueous solution
1Catholic University of America, Department of Biomedical Engineering, 620 Michigan Avenue North East, Washington, DC 20064, USA. yuan@cua.edu
Abstract:
We report experimentally observed ultrasound-modulated fluorescence (UMF) from a submillimeter tube filled with rhodamine B aqueous solution. The tube was submerged in water and a scattering medium. Based on the measured data, we find that the UMF signals might be generated from three mechanisms: modulation of the excitation light, modulation of the emission light, and modulation of the properties of fluorophore. In addition, a linear relationship between the UMF and the drive voltage applied to the ultrasound transducer is found.

