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Related Experiment Video

Updated: Jun 13, 2026

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

Published on: November 22, 2019

Nonlinear microscopy with fiber laser continuum excitation.

Fredrik Svedberg¹, Christian Brackmann, Thomas Hellerer

¹Chalmers University of Technology, Department of Chemical and Biological Engineering, Kemivagen 10, 412 96 Goteborg, Sweden.

Journal of Biomedical Optics

|May 13, 2010

Summary

A new fiber laser enables Coherent Anti-Stokes Raman Scattering/Second-Harmonic Generation (CARS/SHG) microscopy with lower power. This compact, cost-effective system provides comparable biological imaging to traditional methods.

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Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

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Published on: October 28, 2018

Area of Science:

Biophotonics
Laser Technology
Microscopy

Background:

Nonlinear microscopy techniques like CARS/SHG are valuable for biological imaging.
Conventional systems often rely on bulky and expensive optical parametric oscillators (OPOs).
High-power laser sources are crucial for efficient nonlinear signal generation.

Purpose of the Study:

To present a compact, high-power, fiber-based femtosecond laser system for CARS/SHG microscopy.
To quantitatively compare its performance against a conventional picosecond OPO-based system.
To assess its potential for broader adoption in biosciences.

Main Methods:

Development of a compact, high-power, all-fiber femtosecond laser system.
Implementation of the fiber laser for CARS/SHG microscopy.
Quantitative comparison of image contrast, spectral selectivity, and excitation power requirements with a picosecond OPO system.
Imaging of cellular structures like lipid stores, myosin, and collagen filaments in living cells.

Main Results:

The femtosecond laser system achieved imaging at 60 times lower excitation power than the picosecond OPO system.
Despite lower image contrast (2.5x) and spectral selectivity, cellular components were clearly identified.
Images acquired with both systems were visually comparable in information content.
The fiber laser system demonstrated simple operation, a small footprint, and lower cost.

Conclusions:

The fiber-based femtosecond laser system offers a viable, lower-power alternative for CARS/SHG microscopy.
Its advantages in power efficiency, cost, and size promote wider accessibility of nonlinear microscopy in biosciences.
Further development could enhance spectral selectivity and image contrast for specific applications.