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Copy number variation in the bovine genome
João Fadista1, Bo Thomsen, Lars-Erik Holm
1Group of Molecular Genetics and Systems Biology, Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, Aarhus University, Blichers Allé 20, DK-8830 Tjele, Denmark.
This study maps cattle copy number variation (CNV) using high-resolution arrays, identifying 304 regions. These CNVs impact genes related to environmental response and may influence bovine health and production traits.
Area of Science:
- Genomics
- Comparative Genomics
- Mammalian Genetics
Background:
- Copy number variations (CNVs) are key drivers of genetic diversity and disease in mammals.
- The contribution of CNVs to genetic variation in cattle remains largely unexplored.
Purpose of the Study:
- To create the highest resolution map of copy number variation in the cattle genome.
- To identify copy number variation regions (CNVRs) in diverse cattle breeds.
Main Methods:
- Utilized NimbleGen CGH arrays with approximately 6.3 million probes for high-resolution genome tiling.
- Analyzed 20 bovine samples from 4 different dairy and beef breeds.
Main Results:
- Identified 304 CNVRs across the cattle genome, covering 0.68% (22 Mb).
- CNVRs ranged from 1.7 to 2,031 kb, with a median size of 16.7 kb.
- Found that 30% of CNVs encompass genes, many involved in environmental response, with potential links to human disease susceptibility.
Conclusions:
- This high-resolution map of bovine CNVs serves as a valuable resource.
- Facilitates the assessment of CNV impact on cattle health and production traits.
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