Related Experiment Video
Updated: Jun 13, 2026
12:03
Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy
Published on: September 5, 2016
The RNA binding protein HuR does not interact directly with HIV-1 reverse transcriptase and does not affect reverse
Jinwoo Ahn1, In-Ja L Byeon, Sanjeewa Dharmasena
1Department of Structural Biology, Division of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA. jia12@pitt.edu
Retrovirology
|May 13, 2010
Summary
The RNA binding protein HuR does not directly interact with HIV-1 reverse transcriptase (RT) or affect viral reverse transcription efficiency. This study found no evidence of a direct protein-protein interaction influencing HIV-1 replication.
Area of Science:
- Molecular Biology
- Virology
- Structural Biology
Background:
- Previous studies suggested the RNA binding protein HuR interacts with HIV-1 reverse transcriptase (RT).
- HuR, an embryonic lethal abnormal vision protein family member, binds AU-rich elements (AREs) via its RNA recognition motifs (RRMs).
Purpose of the Study:
- To define structural determinants of the HuR-RT interaction.
- To elucidate mechanisms of HuR's influence on HIV-1 reverse transcription in vitro.
Main Methods:
- Cloning and purification of full-length HuR and three RRM constructs.
- Biophysical characterization of HuR proteins.
- NMR titrations to detect protein-protein interactions between HuR and HIV-1 RT (or its RNase H domain).
Main Results:
- All purified HuR proteins were well-structured monomers.
- No direct protein-protein interaction was detected between HuR and HIV-1 RT using NMR.
- HuR did not significantly alter HIV-1 reverse transcription kinetics, even with ARE-containing RNA templates.
Conclusions:
- HuR does not impact HIV-1 replication via a direct protein-protein interaction with viral RT.
More Related Videos
Related Concept Videos
01:29Viruses with RNA Genomes
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
01:20Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...
01:23Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
01:30Inhibitors of Viral Protein Synthesis
Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...
01:25Inhibitors Of Virion Release
Viral replication and dissemination rely on efficient mechanisms for host cell entry, genome replication, assembly, and release. Influenza viruses, such as types A and B, are negative-sense single-stranded RNA viruses with a segmented genome, that depend on two critical surface glycoproteins to carry out these processes: hemagglutinin (HA) and neuraminidase (NA). HA initiates infection by binding to sialic acid residues on the surface of host epithelial cells, facilitating receptor-mediated...
01:19Inhibitors of Virion Maturation and Assembly
As part of their replication cycle, certain viruses synthesize long precursor proteins called polyproteins within infected host cells. In human immunodeficiency virus (HIV), two major polyproteins are produced: Gag and Gag-Pol. The Gag polyprotein supplies the structural components of the virus, while Gag-Pol includes essential viral enzymes such as reverse transcriptase, integrase, and protease. After synthesis, these polyproteins move to the host cell membrane, where they assemble into an...