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Related Experiment Video

Updated: Jun 13, 2026

Viral Nanoparticles for In vivo Tumor Imaging
14:04

Viral Nanoparticles for In vivo Tumor Imaging

Published on: November 16, 2012

Recombinant lambda-phage nanobioparticles for tumor therapy in mice models.

Amir Ghaemi1, Hoorieh Soleimanjahi, Pooria Gill

  • 1Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, 14115-111 Iran. soleim_h@modares.ac.ir.

Genetic Vaccines and Therapy
|May 13, 2010
PubMed
Summary

Recombinant lambda phages efficiently deliver genes into mammalian cells, particularly fibroblasts, and demonstrate therapeutic anti-tumor effects in mice, showing promise as novel nanomedicines.

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Area of Science:

  • Molecular Biology
  • Nanotechnology
  • Immunology

Background:

  • Lambda phages offer a cost-effective and safe alternative to traditional nanocarriers for gene delivery.
  • Limited understanding exists regarding lambda phage gene transfer and expression in mammalian systems.

Purpose of the Study:

  • To evaluate lambda bacteriophage-mediated gene transfer and expression in vitro using mammalian cell lines.
  • To assess the therapeutic anti-tumor potential of recombinant lambda phages as a cancer vaccine.

Main Methods:

  • Constructed recombinant lambda-phage nanobioparticles encoding enhanced green fluorescent protein (EGFP) and human papillomavirus type 16 E7 gene.
  • Transduced four mammalian cell lines (COS-7, CHO, TC-1, HEK-239) with nanobioparticles.
  • Evaluated anti-tumor effects in a C57BL/6 mouse model vaccinated with lambda-HPV-16 E7 phage.

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Main Results:

  • Gene delivery and expression were more efficient in fibroblastic cells (COS-7, CHO) than epithelial cells (TC-1, HEK-239).
  • Internalizing titers were higher in COS-7 and CHO cells compared to TC-1 and HEK-293 cells, despite similar phage entry.
  • Lambda-HPV-16 E7 vaccinated mice exhibited significant therapeutic anti-tumor effects against E7-expressing tumors.

Conclusions:

  • Recombinant lambda phages demonstrate effective gene transduction in mammalian cells.
  • These nanobioparticles show potential for developing novel, safe, and effective phage-based nanomedicines and cancer vaccines.