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Updated: Jun 13, 2026

Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System
Published on: December 21, 2019
[Identification of genes regulated by HBV preS1-transactivated protein 2 binding protein 1]
Huan-hu Zhang1, Dan-qiong Wang, Jun Cheng
1Hepatopathy Center, Second Hospital, Shanxi Medical University, Taiyuan, China.
Objective:
To identify genes regulated by HBV preS1-transactivated protein 2 binding protein 1 (PS1TP2BP1).
Methods:
PS1TP2BP1 gene was amplified by polymerase chain reaction (PCR) technique and cloned into the eukaryotic expression vector pcDNA 3.1/my-c-His A. The mRNAs isolated from HepG2 cells transfected recombinant eukaryotic expression vector pcDNA 3.1/myc-HisA-PS1TP2BP1 and pcDNA 3.1/myc-HisA empty vector were used to construct subtractive library. The differentially expressed genes were identified and analyzed.
Results:
35 differentially expressed clones were obtained. Colony PCR identified 15 clones with 200-1000 bp inserts. Sequence analysis identified 15 differentially expressed genes.
Conclusion:
This study provides data for further characterize the function of PS1TP2BP1.
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