Related Experiment Video
Updated: Jun 13, 2026
A Model of Experimental Steatosis In Vitro: Hepatocyte Cell Culture in Lipid Overload-Conditioned Medium
Published on: May 18, 2021
[Comparison of two nonalcoholic hepatocellular steatosis models]
Hong-ru Wu1, Shao-hua Chen, Yi Lu
1Department of Gastroenterology, the First Affiliated Hospital of College of Medicine, Zhejiang University, Hangzhou, China.
Objective:
To compare two models of nonalcoholic hepatocellular steatosis.
Methods:
HL-7702 cells were incubated with a mixture of of unsaturated oleate acid or 50% fetal bovine serum to induce fat-overloading. Significant fat accumulation was documented by Oil Red O staining , and intracellular triglyceride levels was detected by triglyceride enzymatic assay.
Results:
The results showed that both 0.5 mmol/ml oleate acid and 50% FBS were able to induce nonalcoholic hepatocellular steatosis.
Conclusion:
A nonalcoholic hepatocellular steatosis was induced by 0.5 mmol/ml oleate acid.
