Enzymes responsible for metabolism of Nα-benzyloxycarbonyl-L-lysine in microorganisms

The present paper reviews the enzymes catalyzing conversion of Nα-benzyloxycarbonyl-L-lysine (Nα-Z-L-lysine) to Nα-benzyloxycarbonyl-L-aminoadipic acid (Nα-Z-L-AAA) in fungal and bacterial strains. Aspergillus niger AKU 3302 and Rhodococcus sp. AIU Z-35-1 converted Nα-Z-L-lysine to Nα-Z-L-AAA via Nα-benzyloxycarbonyl-L-aminoadipate-δ-semialdehyde (Nα-Z-L-AASA). However, different enzyme combinations were involved in the Nα-Z-L-lysine metabolism of both strains. A. niger strain converted Nα-Z-L-lysine to Nα-Z-L-AASA by amine oxidase, and the resulting Nα-Z-L-AASA was converted to Nα-Z-L-AAA by an aldehyde oxidase. In the Rhodococcus strain, conversion of Nα-Z-L-lysine to Nα-Z-L-AASA was catalyzed by l-specific amino acid oxidase. The resulting Nα-Z-L-AASA was converted to Nα-Z-L-AAA by an aldehyde dehydrogenase. The present paper also describes characteristics of new enzymes obtained from those strains.