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Rapid identification and subtyping of herpes simplex virus by complement-dependent cytotoxicity
Intervirology
|January 1, 1978
Summary
A new method rapidly identifies and subtypes herpes simplex virus (HSV) in clinical samples. This technique uses specific antisera and staining to detect viral presence within two hours of observing cytopathic effects (CPE).
Area of Science:
- Virology
- Clinical Microbiology
- Immunology
Background:
- Herpes simplex virus (HSV) is a common human pathogen.
- Accurate and rapid identification and subtyping of HSV are crucial for patient management and epidemiological studies.
- Existing methods for HSV detection can be time-consuming.
Purpose of the Study:
- To present a simple and rapid method for identifying and subtyping herpes simplex virus from clinical specimens.
- To reduce the time required for HSV diagnosis.
Main Methods:
- Inoculated cell cultures exhibiting cytopathic changes (CPE) were utilized.
- Type-specific cytotoxic antisera against HSV and complement were applied.
- Cell lysis was detected using trypan blue staining.
Main Results:
- The method allowed for the identification of herpes simplex virus.
- Subtyping of herpes simplex virus was achieved.
- The entire process, from CPE appearance to identification and subtyping, was completed within 2 hours.
Conclusions:
- This technique offers a rapid and straightforward approach for HSV identification and subtyping.
- The method has potential applications in clinical laboratories for timely diagnosis.