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Published on: January 23, 2019
S100A12 on circulating endothelial cells surface in children with Kawasaki disease
Songling Fu1, Fangqi Gong, Chunhong Xie
1Department of Cardiology, Children's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, China.
Insights
S100A12 expression on circulating endothelial cells (CECs) is elevated in children with Kawasaki disease (KD), particularly in those with coronary artery lesions (CALs). This suggests S100A12 may play a role in CAL development.
Area of Science:
- Pediatrics
- Immunology
- Cardiovascular Research
Background:
- Kawasaki disease (KD) is a critical pediatric illness leading to coronary artery lesions (CALs).
- The role of circulating endothelial cells (CECs) and specific biomarkers in KD pathogenesis remains under investigation.
Purpose of the Study:
- To investigate S100A12 expression on CECs in children with KD.
- To explore the correlation between S100A12 expression on CECs and the development of CALs in KD patients.
Main Methods:
- Evaluated CECs to mononuclear cells ratio (CECs/MNC), S100A12 positive rate on CECs, and S100A12 fluorescence intensity (FI-S100A12-CECs) in 42 KD patients across acute, subacute, and convalescent stages.
- Compared KD patients with controls and stratified patients based on the presence or absence of CALs.
Main Results:
- CECs/MNC ratio was significantly higher in acute and subacute KD patients with CALs.
- S100A12 expression (rate and FI) on CECs was significantly elevated in KD patients compared to controls.
- FI-S100A12-CECs decreased in subacute/convalescent KD without CALs but increased in those with CALs.
- Higher CECs/MNC and FI-S100A12-CECs levels were observed in subacute KD patients with CALs versus those without.
- Elevated FI-S100A12-CECs persisted in convalescent KD patients with CALs.
Conclusions:
- S100A12 expression on CECs is significantly increased in Kawasaki disease.
- S100A12 expression on CECs persists longer in patients with coronary artery lesions.
- S100A12 on CECs is a potential biomarker and may be implicated in the development of CALs in KD.
Abstract:
The purpose of this study was to investigate the expression of S100A12 on the surface of circulating endothelial cells (CECs) in children with Kawasaki disease (KD) and the correlations between S100A12 and coronary artery lesions (CALs). The ratio of CECs to mononuclear cells (CECs/MNC), the positive rate of S100A12 on CECs surface (CECs-S100A12/CECs), and the fluorescence intensity of S100A12 on CECs surface (FI-S100A12-CECs) were evaluated respectively in 42 patients with acute stage (A-KD), subacute stage (SA-KD) and convalescent stage KD (C-KD). The CECs/MNC ratio increased significantly in patients with A-KD and SA-KD with CALs. The CECs-S100A12/CECs rate and FI-S100A12-CECs level were significantly higher in patients with KD than in the controls. The FI-S100A12-CECs level decreased to near half levels in patients with SA-KD and C-KD without CALs, but increased continuously in patients with SA-KD with CALs. The CECs/MNC ratio and FI-S100A12-CECs level in patients with SA-KD with CALs were significantly higher than in patients with SA-KD without CALs. The FI-S100A12-CECs level was significantly higher in patients with C-KD with CALs than in C-KD without CALs. The S100A12 expression on the CECs surface increased significantly in patients with KD and persisted for a longer time in patients with CALs, suggesting that the S100A12 expression on CECs may be involved in the development of CALs.
