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Related Experiment Video

Updated: Jun 13, 2026

Frequency Mixing Magnetic Detection Scanner for Imaging Magnetic Particles in Planar Samples

Frequency Mixing Magnetic Detection Scanner for Imaging Magnetic Particles in Planar Samples

Published on: June 9, 2016

Shuttle DNP spectrometer with a two-center magnet.

Alexander Krahn¹, Philip Lottmann, Thorsten Marquardsen

¹Bruker BioSpin, Silberstreifen 4, D-76287 Rheinstetten, Germany.

Physical Chemistry Chemical Physics : PCCP

|May 13, 2010

Summary

This study introduces a new dynamic nuclear polarization (DNP) setup for enhanced Nuclear Magnetic Resonance (NMR) spectroscopy. The novel system successfully hyperpolarizes liquid samples and achieves high-resolution spectra for larger molecules like glucose.

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Area of Science:

Magnetic Resonance Spectroscopy
Hyperpolarization Techniques
Nuclear Overhauser Effect

Background:

Dynamic Nuclear Polarization (DNP) enhances NMR sensitivity by transferring polarization from electrons to nuclei.
Previous DNP setups required separate magnets for polarization and detection, limiting sample handling and applicability.
Improving DNP efficiency and extending its application to larger molecules is crucial for advanced NMR studies.

Purpose of the Study:

To develop and validate a novel DNP setup for hyperpolarizing liquid samples.
To enable high-resolution NMR detection of larger molecules previously difficult to enhance.
To demonstrate the capability of the new setup using specific model compounds.

Main Methods:

A single 14.09 T shielded cryomagnet was modified with a ferroshim system to create a homogeneous low-field region for polarization.
Liquid samples were hyperpolarized via the electron-nucleus Overhauser effect at 0.34 T.
A pneumatic shuttle system transferred the polarized sample to the high-field center (14.09 T) within 40 ms for NMR detection.
A homebuilt EPR cavity and a standard high-resolution inverse (1)H/(13)C selective probe were used for polarization and detection, respectively.

Main Results:

A maximum proton Overhauser enhancement of epsilon(HF) = -3.7 was observed for 4-Oxo-TEMPO-D,(15)N (TEMPONE)/H(2)O.
The new setup successfully enhanced larger molecules, including glucose and 2,2-dimethyl-2-silapentane-5-sulfonic acid sodium salt (DSS), which were not enhanced in previous setups.
High-resolution proton spectra of glucose and DSS in D(2)O were acquired in the high-field position after low-field polarization.

Conclusions:

The developed DNP setup effectively hyperpolarizes liquid samples using a single magnet system.
This approach significantly expands the scope of DNP-enhanced NMR to include larger biomolecules and complex systems.
The demonstrated capability paves the way for new applications in high-resolution NMR spectroscopy.