Related Experiment Video

Updated: Jun 13, 2026

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System
08:54

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Published on: March 20, 2016

Bimodal expression of Sprouty2 during the cell cycle is mediated by phase-specific Ras/MAPK and c-Cbl activities

Christoph-Erik Mayer1, Barbara Haigl, Florian Jantscher

  • 1Department of Medicine I, Institute of Cancer Research, Medical University of Vienna, Borschkegasse 8a, 1090 Vienna, Austria. christoph-erik.mayer@meduniwien.ac.at

Cellular and Molecular Life Sciences : CMLS
|May 13, 2010
PubMed

Insights

Sprouty2 protein levels change during the cell cycle, influenced by Ras and c-Cbl. Its degradation is regulated by c-Cbl, impacting cell proliferation and signal transduction.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Signal Transduction

Background:

  • Sprouty2 acts as a key inhibitor of cell proliferation and signal transduction pathways.
  • Understanding the regulation of Sprouty2 during the cell cycle is crucial for comprehending cell growth control.

Purpose of the Study:

  • To investigate the cell cycle-specific expression profile of Sprouty2 protein.
  • To elucidate the molecular mechanisms regulating Sprouty2 abundance during cell cycle progression.

Main Methods:

  • Analysis of Sprouty2 protein expression during cell cycle progression after quiescence.
  • Investigating the role of Ras-mediated mitogen-activated protein kinase signaling.
  • Assessing the impact of proteasomal degradation and c-Cbl ubiquitination on Sprouty2 levels.

Main Results:

  • Sprouty2 exhibited bimodal expression during cell cycle progression, contrasting with sustained high Sprouty4 levels in G1.
  • Ras-induced mitogen-activated protein kinase signaling was essential for initial Sprouty2 upregulation at the G0/G1 transition.
  • Accelerated proteasomal degradation transiently reduced Sprouty2 during late G1.
  • The second Sprouty2 peak was dependent on c-Cbl ubiquitination, as blocking c-Cbl prevented the decline in Sprouty2 expression.

Conclusions:

  • Sprouty2 abundance is tightly regulated by the coordinated actions of Ras and c-Cbl.
  • c-Cbl-mediated ubiquitination is critical for the cell cycle-specific downregulation of Sprouty2.
  • These findings provide insights into the molecular control of Sprouty2 during cell cycle progression and its role in signal transduction.

More Related Videos

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development09:32

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

Related Concept Videos

MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
Cells Coordinate Growth and Proliferation02:36

Cells Coordinate Growth and Proliferation

Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
Cells Coordinate Growth and Proliferation02:36

Cells Coordinate Growth and Proliferation

Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
The Ras Gene02:38

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a superfamily...