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[Effect of bone morphogenetic protein-7 on aristolochic acid induced renal tubular epithelial cells
Yong-zhi Xu1, Hao Huang, Hua-feng Liu
1Institute of Nephrology, Afiliated Hospital of Guangdong Medical College.
Objective:
To observe the effect of bone morphogenetic protein-7 (BMP-7) on aristolchic acid induced renal tubular epithelial cell trans-differentiation to look for new therapeutic approach for aristolchic acid nephropathy (AAN).
Methods:
In vitro cultured human proximal renal tubular epithelial cell line HK-2 cells were treated with different concentrations of BMP-7 (75 ng/mL, 150 ng/mL and 300 ng/mL) after trans-differentiation of the cells was induced by AA (10 microg/mL). Levels of alpha-SMA mRNA and protein expressions were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blotting respectively.
Results:
BMP-7 reversed the AA inducing alpha-SMA expressions in HK-2 cells in a dose-dependent manner.
Conclusion:
BMP-7 can inhibit the trans-differentiation of human renal tubular epithelial cell induced by AA, thereby might be a new potential drug for AAN prevention and treatment.
