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Related Experiment Video

Updated: Jun 13, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Protein quantitation using isotope-assisted mass spectrometry.

Kelli G Kline¹, Michael R Sussman

¹Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA.

Annual Review of Biophysics

|May 14, 2010

Summary

Heavy isotope labeling in organisms, like nitrogen-15 (15N), offers superior controls for mass spectrometry. This technique enhances the accuracy of quantifying proteome changes in biological research.

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Area of Science:

Proteomics
Biotechnology
Analytical Chemistry

Background:

Cellular proteomes change due to genetic, chemical, and environmental factors.
Mass spectrometry advancements improve measurement accuracy and throughput.
Heavy isotope internal standards are emerging as crucial tools.

Purpose of the Study:

To discuss the theory and practice of using heavy isotope-labeled organisms.
To review recent biological applications of this technology.

Main Methods:

Utilizing mammals grown with near-complete replacement of common elements (e.g., 15N) with heavy isotopes.
Employing isotopically labeled organisms as internal standards.
Applying mass spectrometry for proteome quantification.

Main Results:

Heavy isotope-labeled organisms serve as effective controls.
These controls help isolate and identify experimental variables like extraction efficiencies.
The technology enables more accurate proteome change quantification.

Conclusions:

Heavy isotope labeling is a powerful technique in modern biological research.
It significantly improves the reliability of proteomic studies.
This method has broad applications in understanding cellular responses.