Related Experiment Video
Updated: Jun 13, 2026
Production of High-Yield Adeno Associated Vector Batches Using HEK293 Suspension Cells
Published on: April 26, 2024
Delivery of an EBV episome by a self-circularizing helper-dependent adenovirus: long-term transgene expression in
J S Gil1, S D Gallaher, A J Berk
1Molecular Biology Institute, Department of Microbiology, Immunology and Molecular Genetics, University of California-Los Angeles, 611 Young Drive E, Los Angeles, CA 90095-1570, USA.
Abstract:
Epstein-Barr virus (EBV) evolved an episomal system for maintaining life-long, latent infection of human B lymphocytes. Circular episomes engineered from EBV components required for this latent form of infection have the capacity to persist in most types of replicating mammalian cells without DNA integration and the pitfalls of insertional mutagenesis. EBV episomes are typically transduced using low-efficiency methods. Here we present a method for efficient delivery of EBV episomes to nuclei of hepatocytes in living mice using a helper-dependent adenoviral vector and Cre-mediated recombination in vivo to generate circular EBV episomes following infection. Cre is transiently expressed from a hepatocyte-specific promoter so that vector generation and transgene expression are tissue specific. We show long-term persistence of the circularized vector DNA and expression of a reporter gene in hepatocytes of immunocompetent mice.
