Related Experiment Videos
Hybrid leukemia and the 5q-abnormality.
G Sun1, S Wormsley, R S Sparkes
1Department of Medicine, UCLA School of Medicine 90024-1678.
Leukemia Research
|January 1, 1991
Summary
This study presents a rare case of acute lymphoid leukemia with a 5q- deletion, revealing hybrid features. The findings suggest immature stem cell origin for this unique leukemia subtype.
Area of Science:
- Hematology
- Cytogenetics
- Immunophenotyping
Background:
- Deletions of chromosome 5q (5q-) are typically associated with acute myelogenous leukemia.
- 5q- in acute lymphoid leukemia (ALL) is exceptionally rare, presenting diagnostic challenges.
Purpose of the Study:
- To investigate a rare case of acute leukemia with 5q- using comprehensive techniques.
- To characterize the immunophenotype and cellular origin of this unusual leukemia.
Main Methods:
- Morphologic and cytochemical analyses (FAB L-2, PAS+, MPO-, ASD-).
- Immunophenotyping including TdT, CD5, CD7, and CD13 expression.
- Molecular techniques to assess T beta-cell receptor gene rearrangement.
- Dual staining for antigen co-expression.
Main Results:
- Morphology and cytochemistry were consistent with ALL.
- Leukemia cells expressed T-cell markers (CD5+, CD7+) but lacked T beta-cell receptor gene rearrangement.
- Co-expression of lymphoid (T-cell) and myeloid (CD13) antigens was observed.
- TdT was present, indicating immature lymphoid cells.
Conclusions:
- The 5q- abnormality in this case appears to arise from an immature stem cell.
- This suggests a potential hybrid leukemia with both lymphoid and myeloid characteristics.
- Such cases highlight the complexity of acute leukemia with chromosomal abnormalities.