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Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue

Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue

Published on: August 27, 2016

c-Fos expression during temporal order judgment in mice.

Makoto Wada¹, Noriyuki Higo, Shunjiro Moizumi

¹Department of Physiology, Juntendo University School of Medicine, Tokyo, Japan. m-wada@juntendo.ac.jp

|May 14, 2010

Summary

Mice performing a temporal order judgment task showed increased neural activation (c-Fos expression) in key sensory processing areas. This suggests these brain regions are crucial for deciphering the timing of sensory inputs.

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Area of Science:

Neuroscience
Sensory Processing
Behavioral Neuroscience

Background:

Understanding how the brain orders sensory information in time is a persistent challenge in neuroscience.
Previous research has laid groundwork, but specific neuronal mechanisms remain unclear.

Purpose of the Study:

To investigate the neural correlates of temporal order judgment using a novel behavioral task in mice.
To identify brain regions activated during the processing of sequential sensory stimuli.

Main Methods:

C57BL/6N mice were trained on a temporal order judgment task involving bilateral whisker air-puff stimuli.
Neural activation was assessed by measuring c-Fos expression in brain sections post-task.
Statistical parametric mapping was used to compare c-Fos densities between test and control groups.

Main Results:

Significantly higher c-Fos expression was observed in the test group compared to controls.
Increased activation was found in bilateral primary somatosensory cortex (barrel fields), left secondary somatosensory cortex, and right secondary auditory cortex.
In the primary somatosensory cortex, c-Fos expression was most prominent in layers II and III.

Conclusions:

Temporal order judgment relies on processing bilateral somatosensory information within the primary somatosensory cortex.
Multimodal sensory areas, including the marginal zone, are involved in this temporal processing.
The findings highlight the role of supragranular layers in the primary sensory cortex for sequencing sensory inputs.