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Ultrasensitive detection of antibodies using a new Tus-Ter-lock immunoPCR system

Isabelle Morin1, Nicholas E Dixon, Patrick M Schaeffer

  • 1Comparative Genomics Centre, School of Pharmacy & Molecular Sciences, DB 21, James Cook Drive, James Cook University, Townsville, QLD 4811, Australia.

Molecular Biosystems
|May 14, 2010
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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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