Related Experiment Video

Updated: Jun 13, 2026

Culturing Primary Rat Inner Medullary Collecting Duct Cells
06:11

Culturing Primary Rat Inner Medullary Collecting Duct Cells

Published on: June 21, 2013

Impaired water reabsorption in mice deficient in the type VI adenylyl cyclase (AC6)

Chen-Li Chien1, Yu-Shuo Wu, Hsing-Lin Lai

  • 1Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan.

FEBS Letters
|May 15, 2010
PubMed

Insights

Adenylyl cyclase type VI (AC6) is crucial for kidney function. This study shows AC6 regulates water balance in mice, highlighting its role in water homeostasis.

Area of Science:

  • Nephrology
  • Molecular Biology
  • Physiology

Background:

  • Adenylyl cyclase (AC) type VI (AC6) is a calcium-inhibitable enzyme.
  • AC6 produces cyclic AMP (cAMP) upon stimulation.
  • The specific role of AC6 in kidney function was previously unclear.

Purpose of the Study:

  • To investigate the role of AC6 in kidney physiology.
  • To determine AC6's involvement in water homeostasis.

Main Methods:

  • Utilized two AC6-knockout mouse lines.
  • Performed immunohistochemical staining to localize AC6.
  • Assessed AC activity using forskolin and V2 receptor agonist.
  • Conducted metabolic cage assays and DCE-MRI for water balance evaluation.

Main Results:

  • AC6 was found in the tubular and collecting duct parts of the nephron.
  • AC activity was reduced in AC6-null mice kidneys.
  • AC6-null mice exhibited impaired water homeostasis.

Conclusions:

  • AC6 is present in key kidney structures involved in urine concentration.
  • AC6 plays a critical role in regulating kidney water homeostasis.
  • Targeting AC6 may offer therapeutic potential for water balance disorders.

More Related Videos

Assessment of Kidney Function in Mouse Models of Glomerular Disease09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium08:46

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Published on: September 1, 2015

Related Concept Videos

Aquaporins01:25

Aquaporins

Aquaporins or AQPs are a family of integral membrane proteins whose primary function is to transport water, while some called aquaglyceroporins also transport glycerol. In addition, aquaporins have also been suspected to be involved in transporting volatile substances, such as carbon dioxide and ammonia, across membranes. Such AQPs that act as gas channels are often highly expressed in cells involved in the gaseous exchange, such as red blood cells, epithelial cells, and pulmonary capillaries.
Diabetes Insipidus II: Pathophysiology01:22

Diabetes Insipidus II: Pathophysiology

Normally, water balance is maintained through three interconnected mechanisms: the hypothalamic thirst center, the synthesis and release of antidiuretic hormone (ADH, or vasopressin), and the kidneys' responsiveness to this hormone. ADH is synthesized in the hypothalamus, released from the posterior pituitary, and acts on the distal nephron, allowing water reabsorption and concentrated urine production.Diabetes Insipidus and Its TypesIn diabetes insipidus (DI), this regulatory system is...
Physiology of the Genitourinary System II: Tubular Reabsorption and Secretion01:22

Physiology of the Genitourinary System II: Tubular Reabsorption and Secretion

The kidneys maintain homeostasis through filtration, reabsorption, and secretion. Tubular reabsorption and secretion are crucial in forming urine and regulating electrolytes, water balance, and waste elimination.Tubular Reabsorption and Secretion ProcessesTubular reabsorption is the process that reclaims essential substances such as electrolytes, glucose, amino acids, and water from the glomerular filtrate back into the bloodstream. This is achieved through passive and active transport...
Regulation of Water Intake01:25

Regulation of Water Intake

Osmolality refers to the number of solute particles per kilogram of solvent in a solution. Plasma osmolality specifically indicates the total number of solute particles per kilogram of water in blood plasma. This value reflects the body's hydration status and is tightly regulated through mechanisms controlling water intake and output. While water consumption is a conscious decision, the body has intrinsic regulatory systems to maintain fluid balance. Dehydration, a state of water deficit...