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New classes of Streptomyces coelicolor A3(2) mutants blocked in undecylprodigiosin (Red) biosynthesis
E A Coco1, K E Narva, J S Feitelson
1Medical Research Division, American Cyanamid Company, Pearl River, NY 10965.
Molecular & General Genetics : MGG
|May 1, 1991
Abstract:
Fifteen mutants of Streptomyces coelicolor A3(2) blocked in both the bipyrrole branch (redA) and a second site specific to the undecylprodigiosin pathway were characterized. Some of the mutants were ordered biosynthetically based on cosynthesis experiments. Complementation of each of the mutants with wild-type DNA cloned in low- and high-copy number plasmid vectors allowed the mutants to be separated into 12 new classes which are physically clustered within approximately 37 kb on the S. coelicolor genome. Early-step biosynthetic genes are centrally located and are flanked by later-step and regulatory genes.